N-Acetyl Semax Amidate Dosage Calculator and Chart

N-Acetyl Semax Amidate Dosage

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Dimitar Marinov, Ph.D.

Last Updated January 28, 2024

Semax

Dimitar Marinov, Ph.D.

 January 28, 2024

Researchers studying nootropics may wonder how to determine a suitable N-Acetyl Semax Amidate dosage for their next experiment.

N-Acetyl Semax Amidate is a modified version of the nootropic peptide Semax, with improved stability and potentially prolonged effects.

Based on the available Semax research, N-Acetyl Semax Amidate is expected to have similar nootropic and neuroprotective benefits, including:

  • Improving attention & short-term memory
  • Reducing mental fatigue
  • Protection against ischemia and neurotoxins

Keep reading to discover what researchers must know about this nootropic peptide, including a detailed N-Acetyl Semax Amidate dosage chart.

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Disclaimer: Peptides.org contains information about products that are intended for laboratory and research use only, unless otherwise explicitly stated. This information, including any referenced scientific or clinical research, is made available for educational purposes only. Likewise, any published information relative to the dosing and administration of reference materials is made available strictly for reference and shall not be construed to encourage the self-administration or any human use of said reference materials. Peptides.org makes every effort to ensure that any information it shares complies with national and international standards for clinical trial information and is committed to the timely disclosure of the design and results of all interventional clinical studies for innovative treatments publicly available or that may be made available. However, research is not considered conclusive. Peptides.org makes no claims that any products referenced can cure, treat or prevent any conditions, including any conditions referenced on its website or in print materials.

N-Acetyl Semax Amidate Dosage Chart | Quick Breakdown

Formulation N-Acetyl Semax Amidate Nasal Spray Dosage Injectable N-Acetyl Semax Amidate Dosage
Daily Dosage Start with 600mcg-900mcg (2-3 pumps a day) and increase as needed Start with 500mcg (once daily) and increase to 1,000mcg (once or twice daily)
Cycle Duration Up to 30 days Up to 30 days
Washout Period Equal to the study duration Equal to the study duration
Alternative Regime Up to 60 days at 600mcg/daily Up to 60 days at 600mcg/daily

What is N-Acetyl Semax Amidate?

N-Acetyl Semax Amidate is an acetylated and amidated version of Semax—a peptide developed in Russia and approved as a neuroprotective agent in conditions such as stroke, encephalopathy, nerve atrophy, and more [1].

N-Acetyl Semax Amidate has the same amino acid structure as Semax, which is composed of the Met-Glu-His-Phe fragment from adrenocorticotropic hormone (ACTH) and a Pro-Gly-Pro at the C-terminus [1].

Acetylation and amidation respectively protect the peptide’s N-terminus and C-terminus, making it less susceptible to enzyme degradation and hydrolysis.

As a result, N-Acetyl Semax Amidate possesses the following properties:

  • Passes through the blood-brain barrier (BBB) when administered intranasally or injected, and with potentially enhanced receptor affinity [2, 3].
  • Protects against degrading enzymes such as leucine aminopeptidase and hydrolases [4, 5].
  • Exhibits a 30 min longer half-life in plasma compared to Semax [5].
  • Increased stability in brain tissue, compared to Semax [6].

Let’s now take a look at the mechanisms underlying this innovative research peptide.

N-Acetyl Semax Amidate Dosage

How Does N-Acetyl Semax Amidate Work?

N-Acetyl Semax Amidate is posited to have a similar mechanism to Semax while exerting improved pharmacokinetics and potentially prolonged effects.

As a reference, Semax is devoid of endocrine action but exerts nootropic and neuroprotective effects by passing through the BBB and interacting with various receptors or their messengers, including by:

  • Upregulating the expression and signaling of neurotrophic factors such as brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which have neuroprotective effects and enhance brain cell survival. Studies report a 40% increase in BDNF and a two-fold increase in the expression of the corresponding tropomyosin receptor kinase B (trkB) [7, 8].
  • Downregulating inflammatory and pro-apoptotic pathways in the central nervous system (CNS) such as matrix metallopeptidase 9 (MMP-9), c-Fos), and c-Jun N-terminal kinases (JNKs) [9].
  • Suppressing the degradation of enkephalins, which are the physiological ligands for the opioid receptors. Upregulating enkephalin levels may have a positive impact on mood and reward-related behavior [10, 11].
  • Exerting a positive modulatory effect on the release of serotonin and dopamine in brain regions such as the striatum, as reported in laboratory animal studies [12].

N-Acetyl Semax Amidate Benefits and Research Applications

N-Acetyl Semax Amidate is expected to have similar benefits as Semax, which is used as a nootropic and neuroprotective agent in Russia [1].

While more research is needed, here are some of the most notable benefits observed with Semax thus far:

  • A study in fatigued individuals who received a single 16mcg/kg dose of Semax reported the subjects’ improved accuracy in a memory test, with the Semax group scoring 71% correct responses compared to 41% accuracy in the control group [13].
  • Studies in patients with moderate to severe stroke report significant improvement in BDNF levels and motor function following Semax administration and reduced inflammation during the recovery period [14, 15].
  • A study in 30 patients with alcohol delirium reported that Semax reduced the neurotoxic impact of alcohol poisoning [16].

In addition, Semax may exert benefits outside the CNS, such as by regulating blood flow, microcirculation, and vascular permeability in the gastrointestinal system. The peptide has been cited as a possible therapeutic in settings of peptic ulcer [17].

It is worth reiterating that the above studies were conducted on Semax peptide, and research is yet to confirm these benefits specifically in N-Acetyl Semax Amidate.

N-Acetyl Semax Amidate Dosing Considerations

The main factor affecting N-Acetyl Semax Amidate dosage is the research objective. Yet, research regarding N-Acetyl Semax Amidate administration in clinical settings is lacking.

Therefore, researchers may consider the available research and approved dosages for Semax nasal drops in Russia, varying as follows depending on the indication:

  • Mental fatigue: The 0.1% Semax package insert indicates doses of 200-300mcg, two to three times daily for 3-5 days [18].
  • Optic nerve atrophy: The dosage is 300mcg, given two-three times daily for 7-10 days [18]. Studies have also applied this dosage for up to 30 days with great effectiveness [19].
  • Cognitive impairment in encephalopathy and cerebrovascular issues: The package insert indicates up to 2000mcg applied three to four times daily for 10-14 days. The course may be repeated if needed [18].
  • Traumatic brain injuries: The official dosage is 1400-3500mcg, three times daily for 3-5 days, but the course can be extended for up to 14 days if needed [18].
  • Moderately severe stroke: The package insert of Semax 1% nasal drops recommends 2000mcg-3000mcg administered three to four times daily, with 3-4 hour intervals between each application, for 10 days [20]. Studies in stroke patients have also repeated the protocol after 20 days of washout [14].
  • Severe stroke: For severe cases, the dosage can be increased to 3000mcg-4000mcg administered four to six times daily, at intervals of 2.5–3 hours, for up to 10 days [20].

Researchers may refer to the above Semax recommendations when determining the appropriate N-Acetyl Semax Amidate dosage based on their research objective.

N-Acetyl Semax Amidate Dosage Calculator

Qualified professionals can obtain intranasal N-Acetyl Semax Amidate for research in the form of nasal spray. The most common formulations include 10mg/10ml (0.1%) and 30mg/10ml (0.3%) sprays, both of which deliver 300mcg per dose.

Considering the previously established recommendations for intranasal Semax, here is a dosing protocol for cognitive enhancement research utilizing N-Acetyl Semax Amidate:

  • N-Acetyl Semax Amidate Dosage: Initiate at 600mcg/daily, adjusting the dose according to the individual subject response.
  • Duration of Study: Limit to a maximum of 30 days at 900mcg/daily, and up to 60 days at 600mcg/daily.
  • N-Acetyl Semax Amidate Cycle: Cycle off for a duration equal to the length of the initial study.
  • Notes: Maintain meticulous monitoring of EEG changes and continually evaluate subjects for neurological, psychological, and physiological reactions throughout the study.

N-Acetyl Semax Amidate Administration

In this section, we provide a step-by-step guide to administering N-Acetyl Semax Amidate as a nasal spray in research settings.

Here is how researchers should administer N-Acetyl Semax Amidate nasal spray to test subjects:

  1. Gently clear the research subject’s nostrils to remove any obstructions.
  2. Ensure the nozzle cap is removed and meticulously disinfected using an appropriate antiseptic solution.
  3. Slightly tilt the research subject’s head backward to facilitate optimal spray reach.
  4. Insert the sanitized nozzle gently into one nostril, ensuring comfort and an unobstructed pathway.
  5. Instruct the subject to inhale while ensuring a secure and comfortable nozzle position.
  6. Depress the sprayer, delivering the specified dosage, synchronizing with the subject's inhalation.
  7. After the initial application, shift to the alternate nostril and repeat the aforementioned steps.
  8. Implement a 60-second interlude before administering additional sprays to the same nostril to ensure adequate absorption.
  9. Conclude the procedure by thoroughly disinfecting the nozzle and securely replacing the protective cap, ensuring it is ready for subsequent utilization.

N-Acetyl Semax Amidate has specific storage requirements involving refrigeration at 36-46 degrees Fahrenheit. The peptide should always be refrigerated.

Furthermore, the peptide must not be frozen, heated, shaken, or exposed to direct sunlight, as these factors can destroy the structure of N-Acetyl Semax Amidate and render it inactive.

N-Acetyl Semax Amidate Side Effects and Safety

While N-Acetyl Semax Amidate safety data are lacking, the available research on Semax may help to fill this gap. The latter has been clinically administered in nasal drop form for up to 30 days, and the available research does not report any side effects [21].

Yet, some test subjects may experience side effects related to using nasal spray when receiving N-Acetyl Semax Amidate. These may include mild complaints of discomfort, such as nose and throat irritation.

It is worth noting that one study reported a potential increase in anxiety after intranasal administration of Semax due to its stimulant-like effects. Still, more data are needed to confirm this risk for Semax and N-Acetyl Semax Amidate [13].

Out of an abundance of caution, researchers should limit the co-administration of N-Acetyl Semax Amidate with other stimulants or nootropics.

Researchers planning to use injectable N-Acetyl Semax Amidate may refer to preclinical studies suggesting that injectable Semax is well tolerated by test animals [22, 23]. Subcutaneous injections may lead to typical side effects at the injection site, such as redness, swelling, induration, and pain.

Lastly, elevated BDNF levels have been hypothesized to induce hair loss. Yet, no research substantiates a link between hair loss and N-Acetyl Semax Amidate usage despite its potential to enhance BDNF levels [24].

N-Acetyl Semax Amidate Dosage

Where to Buy N-Acetyl Semax Amidate Online? | 2024 Edition

Researchers and laboratory specialists can legally acquire N-Acetyl Semax Amidate nasal spray from a trustworthy online provider for experimental use.
Selecting a reputable vendor may be difficult, considering the sheer number of available online retailers.

The experts at Peptides.org have consistently had success with Limitless Life’s intranasal peptide products. This vendor is the best choice for those searching for the highest quality N-Acetyl Semax Amidate nasal spray.

Limitless Life

Limitless Life is our favorite supplier of N-Acetyl Semax Amidate nasal spray. The vendor stands out for these reasons:

  • Stringent Third-Party Testing: Limitless Life emphasizes peptide quality and purity, subjecting all of its products to thorough third-party laboratory testing that ensures a minimum purity of 98% for its N-Acetyl Semax Amidate spray.
  • Unmatched Transparency: A detailed Certificate of Analysis is available on the vendor’s website for all of its listed peptides, highlighting their products’ purity and absence of contaminants or fillers.
  • Rapid Dispatch: Thanks to efficient order processing, most orders are shipped the day they are placed. US-based researchers can expect to receive their peptides in as little as two business days with the standard shipping option.
  • Exceptional Customer Experience: The dedicated team at Limitless Life provides steadfast support throughout the purchasing experience, and are available 24/7 by email or phone.

In sum, we trust Limitless Life as our intranasal N-Acetyl Semax Amidate vendor, as they ensure premium quality products and customer satisfaction from start to finish.

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Dosing N-Acetyl Semax Amidate | Verdict

While research on N-Acetyl Semax Amidate may be limited, the peptide appears to have superior stability and effectiveness compared to Semax.

This acetylated and amidated version of Semax is thus expected to offer similar or even enhanced benefits, related to improving cognitive function, protecting the brain against ischemia and neurotoxins, and more!

For high-quality N-Acetyl Semax Amidate nasal spray, visit our top recommended vendor.

References

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  5. Shevchenko, K. V., Nagaev, I. Y., Andreeva, L. A., Shevchenko, V. P., & Myasoedov, N. F. (2019). Prospects for Intranasal Delivery of Neuropeptides to the Brain. Pharmaceutical Chemistry Journal, 53, 89-100.
  6. Shevchenko, K. V., Nagaev, I. Y., Andreeva, L. A., Shevchenko, V. P., & Myasoedov, N. F. (2013). Stability of Semax acetyl to proteolysis in various biological media. Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections, 449, 110–112. https://doi.org/10.1134/S0012496613020166
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  10. Kost, N. V., Sokolov, O. I.u, Gabaeva, M. V., Grivennikov, I. A., Andreeva, L. A., Miasoedov, N. F., & Zozulia, A. A. (2001). Ingibiruiushchee deĭstvie semaksa i selanka na énkefalindegradiruiushchie fermenty syvorotki krovi cheloveka [Semax and selank inhibit the enkephalin-degrading enzymes from human serum]]. Bioorganicheskaia khimiia, 27(3), 180–183. https://doi.org/10.1023/a:1011373002885
  11. Le Merrer, J., Becker, J. A., Befort, K., & Kieffer, B. L. (2009). Reward processing by the opioid system in the brain. Physiological reviews, 89(4), 1379–1412. https://doi.org/10.1152/physrev.00005.2009
  12. Eremin, K. O., Kudrin, V. S., Saransaari, P., Oja, S. S., Grivennikov, I. A., Myasoedov, N. F., & Rayevsky, K. S. (2005). Semax, an ACTH(4-10) analogue with nootropic properties, activates dopaminergic and serotoninergic brain systems in rodents. Neurochemical research, 30(12), 1493–1500. https://doi.org/10.1007/s11064-005-8826-8
  13. Kaplan, A. Y. A., Kochetova, A. G., Nezavibathko, V. N., Rjasina, T. V., & Ashmarin, I. P. (1996). Synthetic acth analogue semax displays nootropic‐like activity in humans. Neuroscience Research Communications, 19(2), 115-123.
  14. Gusev, E. I., Martynov, M. Y., Kostenko, E. V., Petrova, L. V., & Bobyreva, S. N. (2018). Éffektivnost' semaksa pri lechenii bol'nykh na raznykh stadiiakh ishemicheskogo insul'ta [The efficacy of semax in the tretament of patients at different stages of ischemic stroke]. Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova, 118(3. Vyp. 2), 61–68. https://doi.org/10.17116/jnevro20181183261-68
  15. Miasoedova, N. F., Skvortsova, V. I., Nasonov, E. L., Zhuravleva, E. I.u, Grivennikov, I. A., Arsen'eva, E. L., & Sukhanov, I. I. (1999). Izuchenie mekhanizmov neĭroprotektivnogo deĭstviia semaksa v ostrom periode ishemicheskogo insul'ta [Investigation of mechanisms of neuro-protective effect of semax in acute period of ischemic stroke]. Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova, 99(5), 15–19.
  16. Strelets, N. V., & Utkin, S. Y. (2005). P. 6.020 Use of the neurometabolic drug “Semax” for complex treatment of alcohol delirium. European Neuropsychopharmacology, (15), S275.
  17. Ivanikov, I. O., Brekhova, M. E., Samonina, G. E., Myasoedov, N. F., & Ashmarin, I. P. (2002). Therapy of peptic ulcer with semax peptide. Bulletin of experimental biology and medicine, 134(1), 73–74. https://doi.org/10.1023/a:1020621124776
  18. Семакс инструкция по применению: Показания, противопоказания, побочное действие – описание Semax капли назальные 0.1%: фл. 3 мл с крышкой с прилагаемой пипеткой с крышкой, фл. 3 мл с крышкой-капельницей (28676) – справочник препаратов и лекарств. (n.d.-a). [in Russian] Retrieved July 11, 2023, from https://www.vidal.ru/drugs/semax__28676
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  20. Семакс инструкция по применению: Показания, противопоказания, побочное действие – описание Semax капли назальные 1%: фл. 3 мл с крышкой с прилагаемой пипеткой с крышкой, фл. 3 мл с крышкой-капельницей (37577) – справочник препаратов и лекарств. (n.d.-b). [in Russian] Retrieved July 11, 2023, from https://www.vidal.ru/drugs/semax__37577
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  24. Panchaprateep, R., Korkij, W., & Asawanonda, P. (2011). Brain-derived nerve factor and neurotrophins in androgenetic alopecia. The British journal of dermatology, 165(5), 997–1002. https://doi.org/10.1111/j.1365-2133.2011.10514.
  25. Auesomwang C, Maneerattanaporn M, Chey WD, Kiratisin P, Leelakusolwong S, Tanwandee T. Ten-day high-dose proton pump inhibitor triple therapy versus sequential therapy for Helicobacter pylori eradication. J Gastroenterol Hepatol. 2018 Nov;33(11):1822-1828. doi: 10.1111/jgh.14292. Epub 2018 Jun 27. PMID: 29804294.

Scientifically Fact Checked by:

David Warmflash, M.D.

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